Molecular Biology and Gene Transfer Protocols

Andrew Baker and a group of leading experts describe today's most powerful molecular methods for studying the pathogenesis of vascular disease. These detailed, easy-to-follow techniques range from methods that have been successfully used to identify specific mutations involved in cardiovascular disorders to those for transferring genes associated with cardiovascular disease into various vascular cell types in vitro and in vivo. Methods are available to identify novel genes and generate full-length cDNA, easily and efficiently study gene transcription and promoter activity, and accurately determine gene expression levels in individual cell types under various pathophysiological conditions. Vascular Diseases: Molecular Biology and Gene Therapy Protocols offers today's vascular biologist and genetic therapist an unprecedented ability to study the pathogenesis of vascular disease and easily investigate the potential of gene therapies.

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